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Can you describe your experience with molecular biology techniques such as PCR, gel electrophoresis, and DNA sequencing?

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How To Answer This Question?

This question is designed to gauge your familiarity and proficiency with key molecular biology techniques that are fundamental to the role of a Biotechnologist. When answering, provide specific examples of how you have used these techniques in your previous research or projects. Mention any relevant protocols, the purpose of the experiments, and the outcomes. For instance, you might say:

"In my previous role at XYZ Lab, I frequently used PCR to amplify DNA sequences for cloning purposes. I followed standard protocols and optimized conditions to ensure high yield and specificity. Additionally, I used gel electrophoresis to verify the size and purity of the PCR products. One notable project involved sequencing a gene of interest to identify mutations, where I utilized Sanger sequencing and analyzed the results using bioinformatics tools. This experience has equipped me with a solid understanding of these techniques and their applications in biotechnological research."

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